Lecanora darelensis, a new species of Lecanoraceae from Darel Valley, Gilgit Baltistan, Pakistan
DOI:
https://doi.org/10.7751/telopea17708Keywords:
Lecanora, Lecanoraceae, Pakistan, Lichen, New species, Gilgit Baltistan, BiodiversityAbstract
A new species, Lecanora darelensis sp. nov is described here from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of new species within the genus Lecanora. The new taxon is phylogenetically closely related to L. argopholis. The former can be distinguished from latter in having pale yellowish green upper surface (vs yellowish white), larger apothecial disc 0.5–1.5 mm (vs c. 1.2 mm), brown, plane to convex, pruinose to rarely epruinose apothecial disc (vs deep yellowish brown, plane or slightly concave, epruinose), taller epihymenium 25–35 µm (vs c. 20 µm), taller hymenium 110–150 µm (vs 70–75 µm), larger ascospores 14–18 × 5–8 µm (vs c. 12.7 × 7.3 µm), atranorin is detected by TLC (vs atranorin, epanorin, zeorin) and growing on calcareous rocks (vs rock surfaces, detritus, mosses, sloping siliceous and calcareous rocks, boulders, gravel).
References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic Local Alignment Search Tool. Journal of Molecular Biology. 215(3): 403–410. https://doi.org/10.1016/S0022-2836 (05)80360-2
Aptroot A, Iqbal SH (2010) Annotated checklist of the Lichens of Pakistan, with reports of new records. Herzogia 25: 211–229. http://dx.doi.org/10.13158/heia.25.2.2010.211
Bajpai R, Upreti DK, Nayaka S, Dubey U (2012) Lichen flora in and around Mahabaleshwar, Satara district, Maharashtra with Lecanora expallens Ach., as new record to Indian lichen flora. Phytotaxonomy 12: 123–130.
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2): 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Hafellner J (1984) Studien in Richtung einer natürlicheren Gliederung der Sammelfamilien Lecanoraceae und Lecideaceae. Beihefte zur Nova Hedwigia 79: 241–371.
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series Vol. 41. pp. 95–98. (Information Retrieval Ltd: London)
Henssen A, Jahns HM (1974) Lichenes. (G.T. Verlag: Stuttgart)
Iqbal MS, Khalid AN (2024) Diploschistes iqbalii sp. nov. (Ascomycota: Graphidaceae), a new lichen species from Darel Valley, Gilgit Baltistan, Pakistan. Nova Hedwigia. https://doi.org/10.1127/nova_hedwigia/2024/0936
Khan MZ, Khan B, Awan S, Khan G, Ali R (2013) High-altitude rangelands and their interfaces in Gilgit-Baltistan, Pakistan: Current status and management strategies. In Ning W, Rawat GS, Joshi S, Ismail M, Sharma E (eds), High altitude rangelands and their interfaces in the Hindu Kush Himalayas. pp 66–77. (International Centre for Integrated Mountain Development: Kathmandu, Nepal)
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev/mst010
LaGreca S, Lumbsch HT (2001) Three species of Lecanora new to North America, with notes on other poorly known lecanoroid lichens. The Bryologist 104(2): 204–211.
Lücking R, Hodkinson BP, Leavitt SD (2017) The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota – approaching one thousand genera. The Bryologist 119(4): 361–416. https://doi.org/10.1639/0007-2745-119.4.361
Malíček J, Berger F, Palice Z, Vondrak J (2017) Corticolous sorediate Lecanora species (Lecanoraceae, Ascomycota) containing atranorin in Europe. The Lichenologist 49(5): 431–455. https://doi.org/10.1017/S002428291700038X
Martínez I, Aragón G, Lumbsch HT (1999) Lecanora paramerae, a new lichen from Spain. The Lichenologist 31(4): 315–318. https://doi.org/10.1006/lich.1998.0199
Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, LA. pp. 1–8. (IEEE Computer Society) https://doi.ieeecomputersociety.org/10.1109/GCE.2010.5676129
Orange A, James PW, White FJ (2010) Microchemical methods for the identification of lichens. 2nd edn. (British Lichen Society: London) https://britishlichensociety.org.uk/sites/default/files/document-downloads/Orange%20Microchemical%20Methods.pdf
Rambaut A, Suchard MA, Xie D, Drummond AJ (2014) FigTree 1.4.2 software. (Institute of Evolutionary Biology, University of Edinburgh: Edinburgh) http://tree.bio.ed.ac.uk/software/figtree/
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post–analysis of large phylogenies. Bioinformatics 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
Usman M, Dyer PS, Khalid AN (2021) A novel arctic-alpine lichen from Deosai National Park, Gilgit Baltistan, Pakistan. The Bryologist 124(4): 484–493. https://doi.org/10.1639/0007-2745-124.4.484
Vänskä H (1984) The identity of the lichens Lecanora frustulosa and L. argopholis. Annales Botanici Fennici 21(4): 391–402. https://www.jstor.org/stable/23726154
Voytas D (2000) Agarose gel electrophoresis. Current Protocols in Molecular Biology 51: 2–5. https://doi.org/10.1002/0471142727.mb0205as51
White TJ, Bruns T, Lee SJWT, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 18(1): 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Zahlbruckner AV (1926) Lichenes (Flechten). B. Spezieller Teil. Die natürlichen Pflanzenfamilien 8: 61–270.