Morphology and phylogeny reveal Inosperma sorongense sp. nov. (Agaricales: Inocybaceae), from Sorong, Southwest Papua, Indonesia

Atik Retnowati; Wenhua Lu; Helbert Helbert; Supeni  Sufaati; Ida  Haerida; Samantha  Karunarathna

doi:10.7751/telopea22132

Authors

Atik Retnowati National Research and Innovation Agency of Indonesia (BRIN)
Wenhua Lu College of Biology and Food Engineering, Qujing Normal University, and Chiang Mai University https://orcid.org/0000-0001-7283-7596
Helbert National Research and Innovation Agency of Indonesia (BRIN) https://orcid.org/0000-0002-1281-2159
Supeni Sufaati Cenderawasih University, Papua, Indonesia https://orcid.org/0000-0003-4097-3459
Ida Haerida National Research and Innovation Agency of Indonesia (BRIN) https://orcid.org/0000-0001-5769-9383
Samantha C. Karunarathna College of Biology and Food Engineering, Qujing Normal University https://orcid.org/0000-0001-7080-0781

DOI:

https://doi.org/10.7751/telopea22132

Keywords:

Basidiomycota, ectomycorrhizae, taxonomy

Abstract

Inosperma sorongense Retn. & W.H.Lu, a novel species from Sorong, Southwest Papua, is described based on a suite of unique morphological characteristics and unique phylogenetic placement based on analysis of combined ITS and LSU loci. The morphological features of the novel taxon include medium-sized basidiomata with a reddish-orange convex, umbonate pileus, a reddish-orange stipe with a bulbous base, and large basidiospores. Phylogenetically, Is. sorongense sister to Is. misakaense (Matheny & Watling) Matheny & Esteve-Rav. with well support (MLB = 99%, BPP = 1.0). This study provides a full description of the new species, including field photographs of the basidiomata, micrographs, phylogenetic analysis results, and a comparison with similar taxa.

References

Aïgnon HL, Jabeen S, Naseer A, Yorou NS, Ryberg M (2021) Three new species of Inosperma (Agaricales, Inocybaceae) from Tropical Africa. MycoKeys 77, 97–116. https://doi.org/10.3897/mycokeys.77.60084

Aïgnon HL, Fan Y-G, De Kesel A, Bahram M, Ryberg M, Yorou NS (2023) A new species of Inosperma, and first record of I. afromelliolens (Inocybaceae, Fungi) from West Africa. PLoS ONE 18(10), e0290894. https://doi.org/10.1371/journal. pone.0290894

Capella-Gutierrez S, Silla-Martinez JM, Gabaldon T (2009) TrimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25, 1972–1973. https://doi.org/10.1093/bioinformatics/btp348

Crous PW, Jurjević Z, Balashov S, De la Peña-Lastra S, Mateos A, Pinruan U, Rigueiro-Rodríguez A, Osieck ER, Altés A, Czachura P, Esteve Raventós F, Gunaseelan S, Kaliyaperumal M, Larsson E, Luangsa-ard JJ, Moreno G, Pancorbo F, Piątek M, Sommai S, Somrithipol S, Asif M, Delgado G, Flakus A, Illescas T, Kezo K, Khamsuntorn P, Kubátová A, Labuda R, Lavoise C, Lebel T, Lueangjaroenkit P, Maciá-Vicente JG, Paz A, Saba M, Shivas RG, Tan YP, Wingfield MJ, Aas T, Abramczyk B, Ainsworth AM, Akulov A, Alvarado P, Armada F, Assyov B, Avchar R, Avesani M, Bezerra JL, Bhat JD, Bilański P, Bily DS, Boccardo F, Bozok F, Campos JC, Chaimongkol S, Chellappan N, Costa MM, Dalecká M, Darmostuk V, Daskalopoulos V, Dearnaley J, Dentinger BTM, De Silva NI, Dhotre D, Carlavilla JR, Doungsa-ard C, Dovana F, Erhard A, Ferro LO, Gallegos SC, Giles CE, Gore G, Gorfer M, Guard FE, Hanson S-A, Haridev P, Jankowiak R, Jeffers SN, Kandemir H, Karich A, Kisło K, Kiss L, Krisai-Greilhuber I, Latha KPD, Lorenzini M, Lumyong S, Manimohan P, Manjón JL, Maula F, Mazur E, Mesquita NLS, Młynek K, Mongkolsamrit S, Morán P, Murugadoss R, Nagarajan M, Nalumpang S, Noisripoom W, Nosalj S, Novaes QS, Nowak M, Pawłowska J, Peiger M, Pereira OL, Pinto A, Plaza M, Polemis E, Polhorský A, Ramos DO, Raza M, Rivas Ferreiro M, Rodriguez-Flakus P, Ruszkiewicz-Michalska M, Sánchez A, Santos A, Schüller A, Scott PA, Şen İ, Shelke D, Śliwa L, Solheim H, Sonawane H, Strašiftáková D, Stryjak-Bogacka M, Sudsanguan M, Suwannarach N, Suz LM, Syme K, Taşkın H, Tennakoon DS, Tomka P, Vaghefi N, Vasan V, Vauras J, Wiktorowicz D, Villarreal M, Vizzini A, Wrzosek M, Yang X, Yingkunchao W, Zapparoli G, Zervakis GI, Groenewald JZ (2024) Fungal Planet description sheets: 1614–1696. Fungal Systematics and Evolution 13, 183–440. doi: 10.3114/fuse.2024.13.11 https://doi.org/10.3114/fuse.2024.13.11

Deng LS, Yu WJ, Zeng NK, Liu LJ, Liu LY, Fan YG (2021) Inosperma subsphaerosporum (Inocybaceae), a new species from Hainan, tropical China. Phytotaxa 502, 169–178. https://doi.org/10.11646/phytotaxa.502.2.5

Deng L-S, Yu W-J, Zeng N-K, Zhang Y-Z, Wu X-P, Li H-J, Xu F, Fan Y-G (2022) A new Muscarine-containing Inosperma (Inocybaceae, Agaricales) species discovered from one poisoning incident occurring in tropical China. Frontiers in Microbiology 13, 923435. https://doi.org/10.3389/fmicb.2022.923435

Esteve-Raventós F, Larsson E, Pancorbo F, Bizio E, Altés A, Turégano Y, Moreno G, Olariaga I (2024) A taxonomic and phylogenetic contribution on Inosperma Section Inosperma (Agaricales, Inocybaceae) in Europe: Calamistratum and Geraniodorum Groups. Journal of Fungi (Basel) 10(6), 374. https://doi.org/10.3390/jof10060374

Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98. https://www.sciaeon.org/JWB/papers/1999Hall1.pdf

Hu Y, Karunarathna CS, Li H, Galappaththi MCA, Zao C-L, Kakumyan P, Mortimer PE (2022) The impact of drying temperature on basidiospore size. Diversity 14, 239. https://doi.org/10.3390/d14040239

Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20, 1160–1166. https://doi.org/10.1093/bib/bbx108

Kornerup A, Wanscher JH (1967) Methuen handbook of colour, 2nd edn. Eyre Methuen, London.

Kühner R (1980) Les Hyménomycètes Agaricoïdes (Agaricales, Tricholomatales, Plutéales, Russulales). Étude générale et classification. Bulletin Mensuel de la Societe Linneenne de Lyon 49 (no. spéc.), 1–1027.

Lense ON, Wanma JF, Kesaulija FF, Mansyur FI, Rachim AK, Krey K, Wanma B, Kesaulija R, Simanjorang D, Simbiak F (2024) Diversity of flora and fauna in various forest ecosystem types of South Sorong District, Southwest Papua Province. Biodiversitas 25(10), 3884–3898. https://doi.org/10.13057/biodiv/d251049

Li SN, Xu F, Long P, Liu F, Zhang P, Fan YG, Chen ZH (2022) Five new species of Inosperma from China: Morphological characteristics, phylogenetic analyses, and toxin detection. Frontiers in Microbiology 13, 1021583. https://doi.org/10.3389/fmicb.2022.1021583

Matheny PB, Watling R (2004) A new and unusual species of Inocybe (Inosperma clade) from tropical Africa. Mycotaxon 89, 497–503. https://doi.org/10.5962/p.418532

Matheny PB, Hobbs AM, Esteve-Raventós F (2020) Genera of Inocybaceae: New skin for the old ceremony. Mycologia 112, 83–120. https://doi.org/10.1080/00275514.2019.1668906

Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), New Orleans, LA: 1–8. https://doi.org/10.1109/GCE.2010.5676129

Nugroho JD, Mansur I, Purwito A, Suhendang E (2010) Morphological characteristics of ectomycorrhizas on Merbau [Intsia bijuga (Colebr.) O. Kuntze]. HAYATI Journal of Bioscience 17(2), 68–72. https://doi.org/10.4308/hjb.17.2.68

Nylander JAA, Ronquist F, Huelsenbeck JP, Nieves-Aldrey JL (2004) Bayesian phylogenetic analysis of combined data. Systematic Biology 53, 47–67. https://doi.org/10.1080/10635150490264699

Rathnayaka AR, Tennakoon DS, Jones GEB, Wanasinghe DN, Bhat DJ, Priyashantha AKH, Stephenson SL, Tibpromma S, Karunarathna SC (2024) Significance of Precise Documentation of Hosts and Geospatial Data of Fungal Collections, with an Emphasis on Plant-Associated Fungi. New Zealand Journal of Botany 63 (2–3), 462–489. https://doi.org/10.1080/0028825X.2024.2381734

Rehner SA, Samuels GJ (1995) Molecular systematics of the Hypocreales: a teleomorph gene phylogeny and the status of their anamorphs. Canadian Journal of Botany 73, S816–S823. https://doi.org/10.1139/b95-327

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542. https://doi.org/10.1093/sysbio/sys029

Stamatakis A (2014) RAxML version 8, a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313. https://doi.org/10.1093/bioinformatics/btu033

Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Systematic Biology 57, 758–771. https://doi.org/10.1080/10635150802429642

Vaidya G, Lohman DJ, Meier R (2011) Sequence Matrix: concatenation software for the fast assembly of multigene datasets with character set and codon information. Cladistics 27, 171–180. https://doi.org/10.1111/j.1096-0031.2010.00329.x

Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172, 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990

White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ri-bosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Еds) PCR protocols: a guide to methods and applications. Academic Press, New York, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Zhou YL, Deng LS, Yang SD, Liu CF, Fan YG, Yu WJ (2023) Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China. Frontiers Microbiology 14, 1326253. https://doi.org/10.3389/fmicb.2023.1326253