Lithothelium kantvilasii sp. nov. (lichenized Ascomycota, Pyrenulaceae) is described from cool-temperate rainforest in the Tarkine region of north-western Tasmania, Australia. It has a whitish and largely endophloeodal thallus, minute, solitary perithecioid ascomata with an apical ostiole and small, hyaline 3-septate ascospores. A key is provided to the seven Australian species of Lithothelium.
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